Sequence analysis show that 89 amino acids were translated in exon 2 of BoLA-DRB3 and 13.70 % of nucleotide mutated, which resulted in 14.61 % of amino acid change. By contrast, Hae III AA genotype was associated with susceptibility to FMD ( P < 0.01). Hae IIICC and Hae III BC genotype were associated with resistance to FMD ( P < 0.01). By analyzing alleles and genotypic frequencies between healthy and infection with FMD cattle, we found that allele Hae III A was associated with susceptibility to FMD in Wanbei cattle ( P < 0.05), whereas Hae III C was associated with resistance to FMD ( P < 0.01) and may have a strong protective effect against FMD. The results revealed extensive polymorphisms, 6 RFLP patterns were identified. PCR products were characterized by the RFLP technique using restriction enzymes Hae III. One hundred cattle were challenged with FMDV and exon 2 of BoLA-DRB3 genes was amplified by hemi-nested polymerase chain reaction from asymptomatic animals and from animals with FMD. For the further characterization of foot-and-mouth disease virus (FMDV)-induced foot-and-mouth disease, we investigated the association between polymorphism of BoLA-DRB3 gene and FMD resistance/susceptibility of Wanbei cattle challenged with FMDV.
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